Which PCI-E slot do I use for soundcard?

does it matter which gpu slot i use
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microSD card slot for main storage; 40-pin expansion header; Micro-USB NVIDIA uses cookies to enable and improve the use of the website GPC signal

It shows up as an empty slot in the BIOS no matter what graphics card I put in them Windows does not detect any GPU other than the Intel HD I have not put the 390 in the 2nd PCIE slot for obvious reasons Now, did I fry my PCIEx16 slot? Is there any way to fix this? Could this

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